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Reverse genetic approaches utilize genetically engineered mice to analyze the function of disease-associated genes and their variants.
The scaffolds were then implanted into dorsal skinfold chambers of C57BL/6 wild-type mice to analyze their vascularization and incorporation using intravital fluorescence microscopy, histology and immunohistochemistry. Scaffolds seeded with differentiated spheroids exhibited a markedly impaired vascularization.
I have run motor tests in these mice to analyze the progression of the disorder.
Here, we used Jak3−/− mice to analyze the role of Jak3 in CCR7-mediated dendritic cells migration and function.
125I-insulin binding assays were carried out at 12°C on cells from 16-h fasted mice to analyze cell surface binding in the absence of internalization.
Southern blots were performed using genomic DNA from the spleens and bone marrow of the transplanted mice to analyze for integration of viral DNA, using the IRES from the MIG vector as a probe.
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D. S. Pereira et al. used the Per3 knockout mouse to analyze the role of the clock gene Per3 in the circadian rhythm of behavioral responsiveness.
We measured hepatic mNampt and mSirt1 expression levels using a NASH animal model (STAM mice) 7 to analyze whether or not there is an association between the expression of mNampt and mSirt1 and disease progression.
ATRA were dissolved in mouse serum to analyze their inherent distribution.
Secondly, we have utilized immunofluorescence staining of mouse cells to analyze the subcellular localization of endogenous mKIAA proteins.
Due to these characteristics, we have used the E. caproni-mouse system to analyze the factors governing the regulation of immune responses against intestinal helminths and their consequences on the outcome of the infection.
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