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Using neurotensin receptor 1 (NTR1) knockout (KO) and wild-type (WT) mice, these studies evaluated the involvement of NTR1 in the behavioral responses produced by peripheral administration of NT agonists (NT-2 and NT69L).
In mice, these studies have been performed on spleen APCs, because the rates of recovery of APCs from other lymphoid organs and peripheral blood are low.
Since similar demethylated T cells cause lupus-like autoimmunity in mice, these studies indicate that T-cell DNA demethylation is likely fundamental to lupus onset and flares.
Although limited branching analysis was performed in the MMP 7 and MMP 14 transgenic mice, these studies suggest that MMP 3 overexpression is associated with altered branching morphogenesis, while overexpression of MMP 7 or MMP 14 is not.
Despite the finding that the number of HSCs was not different in the bone marrow of ptpn11 +/− and wild-type mice, these studies showed a clear reduced repopulating activity of the ptpn11 +/− HSCs that was associated with diminished HSC self renewal.
This evidence correlates well with important studies on sterile inflammation in the periphery in mice; these studies identify macrophages as the key cell type sensing sterile injury, and provide evidence that they produce IL-1α to elicit the inflammatory response (Chen et al., 2007; Kono et al., 2010b; Rock et al., 2010).
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By mating Noto-cre mice with the conditional R26-lacZ reporter mouse, these studies have traced cells of notochord origin during the dynamic process of notochord formation, elongation, condensation and eventual formation of the NP within the IVD.
Together with indications of left-hemisphere dominance of call perception in mice [65], these studies provide preliminary evidence for similar neuroanatomical and neurophysiological mechanisms mediating the perception of vocalizations in mice and humans.
All mice in these studies were maintained at a National Institute of Allergy and Infectious Diseases or Food and Drug Administration animal care facility and treated in accordance and guidelines of the Animal Care and Use Committee.
There were no adverse side effects observed in nude mice and these studies clearly demonstrate that using PCI as a modality may greatly improve the efficacy of cell-targeted therapeutic agents.
However, long-term stimulation via the TCR showed comparable changes in cells from young or old mice in these studies.
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