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However, during puberty and in the adult virgin mice, the frequency and density of branch points is significantly reduced.
In intravenously immunized mice the frequency went up to 87% ±3.66%).
In IκBα-SR Tg mice the frequency of Treg cells almost reached wild-type levels (Figure S2B, C).
In these mice, the frequency of FoxP3+ cells is low when gating among CD4+6.5+ cells (6.2+0.9%).
However, within C57BL/6 mice the frequency of previously activated CD8+ and CD4+ T cells (CD8+/CD44hi and CD4+/CD44hi) were elevated in high-fat fed mice compared to controls (Fig. 9).
In WT mice, the frequency and total current of mIPSCs were significantly smaller compared to sIPSCs (both P<0.05), but there was no difference in the properties of mIPSCs and sIPSCs in SSADH−/− mice (Table 3).
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In Adv-administrated WT mice, the frequencies of CD69+ cells in CD8+ T cells in the iLNs were increased, while those in the MLN were not.
However, in the case of Adv-administrated IFNAR2 KO mice, the frequencies of CD69+ cells in CD8+ T cells in the iLNs were significantly reduced compared with those of Adv-administrated WT mice.
Similar to what we found in young mice, the frequencies of LT-HSCs, ST-HSCs, MPPs, and LSK population were not significantly different between aged WT and Smurf2-deficient mice (Fig. 2E).
Transplantation of K14-mOVA lymph nodes did not affect the endogenous pools of CD8+ T cells, CD4+ T cells, or CD4+FoxP3+ Tregs, as in the transplants as well as in the endogenous lymph nodes of the different 'chimeric' mice, the frequencies of the various T cell subsets were comparable.
In spleen cells of mice adoptively transferred with cells from NP-mouse the frequency of CD4+ and CD8+ T cells originating from NP-mice following antigenic stimulation in vivo with Ag85B protein in the presence of anti-4-1BB mAb or control IgG2a mAbs was analyzed by gating lymphocytes positive for CD45.2.
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