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In the null mice, the area of type-X collagen immunostaining, a marker of the hypertrophic zone in cartilage, was slightly smaller than that of wild-type animals at E18.5 and after 4 weeks of postnatal development.
While we did not observe overall loss of total articular cartilage in gefitinib-treated mice, the area and thickness of uncalcified cartilage were remarkably decreased in those mice compared to their vehicle controls.
Importantly, in NOS3−/− mice, the area of vessel wall enhancement remained higher 15 and 30 days after injury compared with that observed in WT mice at the same time points.
In contrast, in the 3 × Tg-AD mice the area density of Mac-1-IR (total activated microglial cells) was significantly increased throughout the whole extension of CA1 at 12 months (111% P=0.0311) and 18 months (88% P=0.0330), but showed only a non-significant rise at 9 months (55.44%, P=0.1163).
Thus, in WD-fed DKO mice, the area of the innominate artery affected by atherosclerotic lesions was strikingly reduced compared to ApoE-KO controls (Fig. 2 A, C ). WD-fed 11β-HSD1+/− (het) mice also had reduced atherosclerotic lesions at the innominate artery, showing that partial 11β-HSD1 defisiency is also protective against severe atherosclerosis (Fig. 2 A).
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For each mouse, the area of 50 randomly chosen adipocytes was measured in five representative sections using Image J software (National Institutes of Health, USA) at 10× magnification.
Deer and antelope and mutant black-furred field mice inhabit the area.
Skin thickness was measured on the backs of the mice in the area of intradermal injections 1 day before killing.
Ascending aortic areas of saline-infused genotypes were between 14 and 15 mm, while AngII infusion in CCR2+/+ mice increased the area to 24.6±0.8 mm.
The 110 mg/kg dose of vancomycin is an effective dose in mice, approximating the area under the curve (AUC) of 400 mg.hour/L for a typical human dose of vancomycin (1 g every 12 hours) [ 20, 38].
In wild-type mice, the median area of the droplets was 4.3 μm and fewer than 5% were greater than 22 μm.
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