Mice sensitised to CII showed a statistically significant decrease in whole body BMD as compared to age-matched control mice 55 days post-sensitisation.
Female BALB/c mice were systemically sensitised with ovalbumin (OVA) and chronically challenged with low mass concentrations of aerosolised OVA for up to 6 weeks.
This is of interest because we have previously shown that although these animals are not systemically sensitised, there is low-level sensitisation via the respiratory tract as a consequence of the chronic challenge, leading to a specific humoral immune response [ 16].
Following short term as well as long term OVA challenges in OVA sensitised mice, AHR of comparable magnitude developed.
Control mice were sham sensitised using an equivalent volume of PBS/Alum.
Sensitised mice were exposed to prolonged ovalbumin challenge or continuous house-dust mite exposure to induce chronic inflammation and remodelling.
Importantly, no adverse effects were seen in muscle of wild-type mice injected systemically with scAAV.hSGCB.
Oral administration of murine IFN-γ significantly elevated the survival of mice challenged systemically with a lethal dose of encephalomyocarditis virus (Tovey and Maury, 1999).
However, one caveat to this analysis is that these mice are systemically deficient in IFNAR1.
In contrast, none of the mice receiving systemically applied antiretroviral PrEP exhibited viral DNA in tissues (Figure 3D; Table 1).
