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Accordingly, EM analysis of kidneys from these mice revealed normal configuration of glomerulus (Fig 3Bi).

Furthermore, immunohistochemical (IHC) analysis of spleen sections from TRAF6-ΔB mice revealed normal splenic architecture, with typical T cell areas surrounded by follicular and MZ B cell areas, separated by MOMA1+ metallophilic macrophages (Fig. 3c).

Limited cognitive/behaviour testing of Stx1a−/− mice revealed normal performance in the open field and Morris water maze tests.

Analysis of pro-T cells within the C57BL/6 and FVB/N mice revealed normal levels of proliferation (Fig. 7F), suggesting that reduced numbers were not due to differential expansion in situ, but rather due to reduced recruitment.

For instance, Rac2-deficient mice revealed normal thymocyte development or positive/negative selection, and only impaired actin polymerization, Ca2+ generation, and Erk and p38 activation during T-cell stimulation and Th1 differentiation [ 21].

At 14 15 months of age, G2019S-LRRK2 mice reveal normal levels of dopamine, DOPAC and HVA and normal dopamine turnover in the striatum and cerebral cortex (Figure 4A D).

G2019S- and R1441C-LRRK2 transgenic mice reveal normal numbers of TH+ dopaminergic VTA neurons at 19 21 months despite detectable expression of G2019S-LRRK2 mRNA in VTA neurons (Figure 3F and S2).

(C ) H&E stained brain sections of wild-type, Nde1−/−, Nde1+/−Trp53−/−, and Nde1−/−Trp53−/− mice reveal normal size and anatomical structure of the Nde1−/−Trp53−/− brain.

Scanning electron microscopy (SEM) analysis of 6-month-old wild-type mouse lens revealed normal overlapped fiber cell layers forming three equally long suture branches as a "Y" pattern at the lens pole, which is a characteristic of mice, rats, guinea pigs, cats, dogs and cows lenses [ 44].

Analysis of lateral dopaminergic fibers that project rostrally to the striatum in the Nestin-Smo cko mice at E13.5 revealed normal projections (Figures 3B 3C).

Indeed, H&E stained sections of the inner structure of liver from Alb-Cre-Fxn Alb-Cre-Fxn Alb-Cre-Fxna normaL3/Lver organization, with hepatocyteS displaying normicefeaturevealedompared with conormalmice (Fig. 2F,G and supplivertary material Fig. 2C).