Each study was designed to use the minimum number of mice required to obtain informative results (meaning quantitative data amenable to statistical analysis) and sufficient material for subsequent studies such as cytokine determination, immunohistochemistry, and expression profiling.
Collectively, the data suggest that tumor formation in NG2-Cre Lgl1 KO Ink4a/Arf+/− mice required inactivation of p19Arf expression, hence the long latency (Supplementary Fig. 5).
Fourteen months old tg-ArcSwe mice required a longer time to re-habituate to the IntelliCages than non-tg mice.
Previously, the best existing treatment for Alzheimer's disease in mice required several months to reduce plaque in the brain".
The technique was performed under light general anesthesia, permitting the individual mice to recover, and thereby minimizing the number of mice required for a time-course study.
To restore lower β-cell proliferation rates to diabetic NOD mice required several days of insulin-induced euglycemia (Figure 6, pellet, late).
In agreement with this late requirement, a complete rescue of Pdx1 expression and endocrine pancreas differentiation in Raldh2−/− mice required RA treatment until E 9.5 [21], [21].
Importantly, as expected, the 21-month-old wild-type mice required more time than the younger controls to find the hidden platform in a Morris water maze.
Starting immediately from 2 sec, mice required more days to reach criteria compared with previous phases.
Nude mice required a rather higher dose of the priming agent, Propionibacterium acnes, than heterozygote littermates.
