As in mice, the reporter expression was maximal on day 3 4 but declined over time more rapidly than in mice and was completely absent by day 10 (not shown).
Our data provides a carefully detailed analysis of endogenous Wnt signaling in the intestine of Wnt-reporter mice and corroborates reporter expression with nuclear β-catenin staining.
We therefore generated transgenic mice and examined reporter expression in the heart at postnatal day 3. Again, we found no expression in cardiac tissues (Additional file 2).
We generated stable DR5-TA mouse lines from the mouse ES cells and analyzed reporter expression in live embryos.
Then, we annotated the same anatomical terms as per the enhancer assays, which allowed 4-way comparisons: zebrafish endogenous gene expression (Zg), mouse endogenous gene expression (Mg), zebrafish transgenic reporter expression (Zt) and mouse transgenic reporter expression (Mt) (Additional file 3).
Interestingly, our detailed analysis revealed that the Tg-4x150 mice show higher and more specific reporter expression than that of the Tg- 10kb mice.
The Ciona CiHox1 (1UP1.4) and CiHox3 (1CiHox3) regulatory sequences when tested in mouse and chicken embryos generate reporter expression in rhombomere 4 in a manner similar to the mouse mb1, mb2 and ma2 enhancers (Table 1).
The preliminary results indicate that the effects of in vivo pancreas transduction in BALB/c mice were similar as in CD1 mice, with a high level of reporter expression seen on day 4 and less on day 7, while areas of the transduced pancreas contained inflammatory cells at both time points.
We have recently demonstrated that Runx2 interaction with a 150-bp mouse Col10a1 cis-enhancer is required but not sufficient for its hypertrophic chondrocyte-specific reporter expression in transgenic mice, suggesting requirement of additional Col10a1 regulators.
Finally, only 8/47 (17%, containing on average 2.3 expression domains in zebrafish and 2.3 in mouse) sequences showed fully consistent reporter expression patterns (Additional file 1).
