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The vascularized engineered muscle tissue implanted into a full-thickness defect in immunodeficient mice remained viable and anastomosed with the host vasculature within 9 days of implantation.
However, most tumor nodules in mice remained viable, and any responses seen by MRI appeared to be transient.
A study reported by Ahituv et al. [45] showed that after deleting four non-coding ultra-conserved elements from the mouse genome, the mice remained viable and fertile, and revealed no abnormalities for a variety of phenotypes that were studied.
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In this study, mouse kidney slices remained viable during the incubation period of 72 h, as determined by ATP content, a parameter generally used as indicator of viability.
MBD2-null mice developed normally and remained viable and fertile, although MBD2-null mothers failed to nurture their pups properly (Hendrich et al. 2001).
According to the killing efficiency estimated in spore-killing experiments (approximately 25%, Fig. 4B), around 7.5×106 CFU spores should be remained viable, and theoretically the mortality of mouse should have reached up to 100% (Fig. 5A, 7.5×106 CFU groups).
They sacrificed some of the engineered mice and isolated a slice of the light-sensitive somatosensory cortex, which remained viable for several hours.
Perhaps just as striking, but overshadowed at the time, was the observation that in the only adult mouse injected with the same NGF antiserum, 34% of sympathetic neurons remained viable versus control.
Interestingly, when cultured ex vivo, RIPK1 KO hematopoietic stem cells remained viable and differentiated normally, supporting the view that the loss of hematopoietic progenitors in RIPK1 KO mice is a consequence of a RIPK3-dependent systemic inflammation.
Furthermore, 95.2±3.2% cells remained viable.
Five tissue blocks remained viable for immunohistochemical analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com