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While prostates of mice receiving standard chow were enlarged and burdened with poorly differentiated carcinoma, those of mice on the supplemented diet appeared normal.
BALB/c mice, which are immunocompetent, showed acceleration of M.tb clearance achieving apparent sterilization after 16 weeks of adjunctive tofacitinib therapy at average exposures higher than recommended in humans, while mice receiving standard treatment alone did not achieve clearance until 24 weeks.
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One group of mice received standard laboratory chow (5% fat— RD) and served as a normal control.
For intergenerational analysis, we randomly seleced 12 F1 males and 12 femalesles per treatment group on PND21; these mice received standard chow without DEHP (Altromin).
One week later, animals were stratified into two groups: one group (25 mice) received standard control diet (Ssniff, Soest, Germany), the second group (24 mice) was fed a modified diet (Ssniff) containing 0.1% wt/wt atorvastatin (Ca2+ salt, catalog no. A2476; TCI, Tokyo, Japan).
* PF-4 Serum obtained at the end of the study period from Lady mice receiving a standard diet alone or an E/S/L-supplemented diet were subjected to a standard biomarker discovery protocol according to Materials and Methods.
Group 2 was a positive control group with 40 mice receiving the standard WHO regimen for DS TB i.e. 2 months of rifampin (R) isoniazid (H) and pyrazinamide (Z) followed by 4 months of R and H (abbreviated as 2RHZ/4RH).
Serum profiling was therefore restricted to compare Lady transgenic mice receiving a standard diet with those receiving E/S/L-supplementation, as the latter group alone clearly exhibited the benefits.
Those receiving standard of care deteriorated.
The mice received a standard diet with free access to tap water.
The mice received a standard mouse feed (ssniff® R/M-H, V1535-300, Soestf, Soest, Germany).
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