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Naïve mice received pooled sera collected from different groups, as indicated in Figure 4C, in a 1∶100 dilution, by intraperitoneal route, 2 h before challenge.
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Control mice received sera pooled from control FVB-huHER2 mice (untreated or pVAX1-treated).
Groups of NOD/Scid mice received DNCD3 splenocytes (5×105 cells/mouse) from a pool of 14 day-old NOD or NON.NOD control females at the same time with diabetogenic splenocytes (5×105 cells/mouse) from 4 5 month-old hyperglycemic NOD mice.
Young mice received blood from older mice, and old mice received blood from younger ones.
Then some mice received large doses of quercetin for a week; others received a placebo.
Control mice received saline only.
Control mice received PBS only.
Prior to imaging mice received lucefirin (0.5 mg/mouse, intraperitonially).
Control mice received 177Lu-DOTA-control IgG.
An additional 20 mice received unlabeled MX35.
Sham mice received a noncoding plasmid.
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