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A weight loss exceeding 25% in all infected mice was used as the experimental end-point, and mice reaching this end-point were euthanized according to IACUC guidelines.
All mice reaching this stage were culled in accordance with Home Office guidelines (Fig. 1I).
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We found that tumors in the control mice reached this point within 7 weeks, while none in the shRNA mice did even by 13 weeks.
Similarly, NVP-LDE225-treated mice reached this same tumour size slightly later, on day 84, 7 weeks after the end of the treatment.
At this age, mice reach the critical period of Aβ plaque formation where insoluble Aβ42 peptides increase five-fold compared to younger animals and the first scarce small plaques can be seen in hippocampus and neocortex.
IMO-treated mice reached the tumour size of 2 cm on day 98, 10 weeks after treatment withdrawal, whereas mice treated with everolimus did not reach this size until the end of experiment, on day 119.
Mecp2−/y-EE mice reached the platform significantly faster than the control Mecp2−/y mice (figure 2D).
In contrast, only 24±8% of AL and 13.4±6.3% of SO mice reached the same criterion during the first session.
Forty-one of 43 mice reached the established criteria.
The Intervention trial was initiated when Rip1-Tag2 mice reached the age of 10 weeks.
Under photopic conditions, trained wild-type mice reached the visible platform in ∼10 s.
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