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The DMD-null mice produced from the DMD-null ES cells were viable but displayed severe muscular hypertrophy and dystrophy.
The DMD-floxed mice produced from the DMD-floxed ES cells were viable, phenotypically normal, and were born with the expected Mendelian frequency, despite the absence of brain (cortical -type dystrophin (Dp427cortical -type
Control mice were wild-type (WT) littermates of INI mice, produced from heterozygous female/hemizygous male matings.
An alternative is to use deep sequencing of PCR-amplified target loci from founder mice produced from different experiments.
In this study we use RNAseq to explore allele specific expression (ASE) in adipose tissue of male and female F1 mice, produced from reciprocal crosses of C57BL/6J and DBA/2J strains.
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These mice produce from four months of age natural anti-Gal antibodies as humans without the need of any additional Gal antigen exposure.
Chimeric mice were produced from mouse ES cell lines.
Interestingly, significantly higher percentages of γδ T cells from Tg mice produced IL-17 than those from Wt mice, especially CD44hi γδ T cells.
(68) They found that LPS-pretreated RPMs from these mice produced lower quantities of PG-Gs from exogenous 2-AG than RPMs from wild-type mice, again supporting a predominant role for COX-2 in the case of exogenously provided endocannabinoid.
Moreover, splenocytes derived from CII/CFA-immunised mice produced more RANKL than those from naive mice.
In AdC68NP-immunized mice, NP-specific CD8+ T cells from old mice produced mainly IFN- γ only; those from young mice had a higher portion of cells that produced IFN- γ together with TNF- α. Figure 3(b) show percentages of the 7 different combinations of cytokine production profiles).
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