Neither SLE nor control BM mDCs produced detectable IFN-α.
EDTA-treated whole-blood samples from these patients were injected intraperitoneally into mice, and the two samples positive by microscopic examination also produced detectable levels of spirochetemia in mice.
The antler velvet sample from elk 01-0306, which produced detectable amplification products after 3 rounds of PMCA, caused disease in Tg(CerPrP 1536+/– mice (mean incubation time 442 d, attack rate 75%) and in Tg(CerPrP-E226 5037+/– CerPrP-E226 5037+/
None of the cultures produced detectable levels of IL-4.
Notably, no methanol-grown strains produced detectable 1-butanol.
Nonetheless, using a highly MSH2‐dependent mechanism, Ung −/−Smug1 −/− mice can still produce detectable levels of switched isotypes, especially IgG1.
These results suggest that Ad vector administration at the neonatal stage does not induce immune tolerance against the Ad vector, although neonatal mice do not produce detectable levels of anti-Ad antibody following the first injection of an Ad vector.
In vitro assays indicated that isolated IsdB specific splenocytes from immunized mice produced abundant IL-17A, much less IFN-γ and no detectable IL-4.
We then measured their cytokine profile and show that CD8+ T cells from healer CBA mice produced more IFN-γ as compared with nonhealer BALB/c mice (Fig. 5); no IL-4 or IL-10 was detectable (data not illustrated).
However, OVA immunized mice produced OVA-specific IgG, as micro-titre wells coated with OVA, but not with MOG, showed bound IgG which was easily detectable using ELISA.
