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The concentrations of DTX in tissue samples were determined by the HPLC method as same as the analysis of the mice plasma samples.
All the analyses with a flow rate of 1.0 mL/min for the mobile phase, the retention time of DTX in rat plasma samples and mice plasma samples were approximately 10.7 and 11.2 min, respectively.
The rat plasma samples employed the mobile phase consisted of HPLC grade acetonitrile, deionized water, tetrahydrofuran, ammonium hydroxide solution (25%), and acetic acid solution (36%) [55:45:3:0.03:0.06 (V/V)]; the mice plasma samples employed the mobile phase consisted of HPLC grade acetonitrile, deionized water, and tetrahydrofuran [55:45:4 (V/V)].
This typical cycle of nadir and peak concentrations is very much in accordance with published data on laboratory rats (plasma samples [38], [39]; faecal samples [40]) and mice (plasma samples [41] [43]; faecal samples [31], [44], [45]).
In accordance with the in vivo assessment of intestinal permeability, LPS levels were significantly lower in Ob-Pre mice plasma samples, as compared to the other groups (fig 2B).
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Mouse plasma samples (2 µl) were fractionated by SDS-PAGE using 4% gels.
To measure Timp1, we diluted mouse plasma samples 1∶6, while a 1∶400 dilution was used for Lcn2.
In order to investigate the possibility of microbial symbiotic origin of DING proteins in eukaryotes, we analyzed wild-type and germ-free mouse plasma samples using western blot assays.
In summary, mouse plasma samples of 20 μl were diluted with 180 μl of human plasma.
Mouse plasma samples were tested for the presence of anti-FIX Abs by ELISA, as described (Follenzi et al, 2004).
Mouse mast-cell protease-1 and histamine were determined on mouse plasma samples by ELISA (eBioscience or Immunotech, respectively), according to manufacturer instructions.
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