We infected bone marrow-derived dendritic cells (BMDCs) from WT C57BL/6J mice or from IRF3-knockout (Irf3−/−) mice with WT and NS1 mutant viruses.
In a follow-up study, two conditional RIPK1 KO mice were generated, in which RIPK1 was deleted from adult mice or from hematopoietic cells.
The increase in plasma leptin appears to result in part from upregulation of adipose leptin secretion (e.g., in mice), or from production and secretion of leptin from the placenta (e.g., in humans and some bats).
The homogenate samples (whole organ in 1 ml of PBS) of control mice or from animals infected with C. neoformans, as well as the serum samples from infected animals or from patients with diagnosed cryptococcosis, were stored at −80 °C until assayed.
In vivo, 4 week old vein graft sections from WT and Eph-B4 knockout mice, or from Ephrin-B2/Fc stimulated or unstimulated mice, were stained with Masson's Trichrome, and the total area of blue staining surrounding the vein graft was quantified to determine graft wall collagen content.
Anti-Sm precursors were present in a frequency of approximately 1 per 10-30,000 in spleen cell cultures from anti-Sm positive mice, but were undetectable when spleen cells from serologically negative MRL-lpr mice or from normal mice were examined.
Recently, we characterized volatile components released from the body of C57BL/6 mice or from their urine [26].
MSCs from C57BL/6 or DBA/1 mice or from iNOS- or IL-6-knock-out C57BL/6 mice were isolated from bone marrow (BM).
In our recent studies [7], [12], mouse embryonic fibroblasts (MEFs) with dysfunctional telomeres derived from mTERC−/− mice or from PARP-1−/− mice displayed differential expression of genes involved in inflammatory response [5], [6], [13].
Negative control images (with omission of the primary antibody) and istotype control images for lung sections taken from either saline control mice or from mice exposed to HDM for 15 weeks (as indicated) are shown in Fig. S1.
Primer sequences were given in the table 5. Q-PCR was performed on total RNA isolated from liver of individual mice or from individual LCLs in a Lightcycler system (Roche Diagnostics).
