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We found that there were fewer myonuclei in lmna-null mice, of which ∼50% had morphological abnormalities.
This study included 4 groups of 19 mice, of which 8 were used for electrophysiological and behavioral experiments.
The first aimed at counting and phenotyping circulating leukocytes during the first parasitaemic wave (n = 24 mice, of which 12 controls were mock-inoculated).
Viral replication was also observed in C57BL/6 mice, of which 2 young and two adult mice were infected (data not shown).
Negative control group 1 consisted of 40 infected and untreated mice, of which 10 were killed the day after infection (D-18), 20 at beginning of treatment (D0), and 10 kept untreated for assessing mortality from tuberculosis.
Such an embryonic lethal phenotype was not rescued in ΔT mice, however, unlike CXCR4-null mice of which only 50% survived till E17.5 and all with reduced body sizes [4], ΔT embryos were present at Mendelian ratios till E18.5 with grossly normal body sizes and morphology (data not shown).
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We used four different types of wild-type controls and mutants including Pcd mice, L7-Pp2b mice, L7-Δγ2 mice and α6-Cacna1a mice, all of which had a C57BL/6 background.
There are four subspecies of wild house mouse, of which the two most prominent are Mus musculus, which evolved in Western Europe, and Mus domesticus, which came from Eastern Europe.
For the pregnant groups, four placentas and 4 fetuses were also collected from each pregnant mouse, of which half were frozen immediately and the other half were fixed in formalin for further study.
14 dysferlin protein protein interactions are recorded in STRING for human and mouse, of which 6 are direct interactions described in PubMed references (CAV3, CAPN3, AHNAK, PARVB, ANXA1, ANXA2).
This is part of the International Knockout Mouse Consortium (IKMC) (22) that aims to generate mutants for all of the protein-coding genes in mouse of which WTSI is a member.
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