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In the present study, HAB and LAB mice of generations 18 22 and NAB mice of generations 1 3 were used.
In the present study, HAB and LAB mice of generations 12 23 and NAB mice of generation 1 were used.
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Our dataset consists of genotypes of all autosomes from 96 mice of generation G2 I5 to G2 I12.
Hundreds of mice of various generations were genotyped, but on average only few are from the same line.
The usefulness of TOKMO-3 seed mice for generation of tissue-specific promoter driven transgenes is now under investigation and has already been verified for some promoters using TOKMO-1 model, the first generation seed mouse (data not shown).
In brief, rhBMP2 (Infuse, Medtronic, TN) molecule was used as immunogen in mice for generation of mAbs.
When mice of the younger generations were crossed with those of a separate and unrelated strain, the methylation and the silencing disappeared in their offspring.
Heterozygous mice of the F4 generation were then mated to obtain homozygous Ugt1−/− mutant mice.
Pufnstuf was the Mickey Mouse of my generation.
As van Dreunen put it, Nintendo has created "the Mickey Mouse of this generation".
At least five mice per strain of generation N3 or later were tested for transgene copy number variation with no significant differences obtained (data not shown).
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