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Source inocula as well as mice negative for PrPCWD by both WB and IHC were further analyzed by PMCA.
Primary astroctye cell cultures prepared from perinatal STAT3-CKO mice grew well under standard conditions and had an appearance under phase-contrast microscopy similar to that of cells from littermate control mice negative for Cre expression.
Electrophysiology was performed in a total of eight VGAT-ChR2 positive mice, six VGAT-Cre mice with viral injections, and three mice negative for ChR2 (total = 17 mice).
Sample or animal exclusion The pre-established criteria to exclude animals from entire study are the mice negative for viral load in liver at the time of assay, or death of fulminant hepatitis or unkonwn reasons.
This group demonstrated that CCR1 may have anti-inflammatory functions since mice negative for CCR1 showed enhanced Th1 immune responses and worsened histology in a model of nephrotoxic serum nephritis [ 39].
Only those mice negative for the Cre allele were used for backcrossings to C57BL/6NCrl, 129S6/SvEvTac, or CD1 and further analyses were carried out with Fltp ZVΔneo/+ mice backcrossed several generations in C57BL/6NCrl, 129S6/SvEvTac, or CD1.
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Results for the B. miyamotoi sensu lato ELISA were positive for all 10 B. miyamotoi sensu lato?"infected mice and negative for all 10 uninfected mice.
IF staining confirmed the co-localization of the murine Fpr2 with α-smooth muscle actin in aortic SMCs isolated from Fpr2+/+ mice, whereas those obtained from Fpr2−/− mice stained negative for the Fpr2 protein.
Pancreatic ducts and acini from control mice and early-stage PanINs from KPC mice were negative for fascin, but approximately 6% of PanIN3 and 100% of PDAC expressed fascin.
The majority of BALB/c F9−/Y mice were negative for IFN-γ, whereas most of the C3H/HeJ F9− / Y mice had some level of IFN-γ (Fig. 4b).
Also, the lung lesions from FoxM1b Tg Arf+/− mice were positive for albumin-mRNA, while the lung samples from the Arf−/− mice were negative for albumin mRNA (Fig 1E).
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