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So these results in germ-free mice need to be confirmed in humans.

Further increasing the yields of correctly folded rOmpF by addition of hydrophobic agents such as ethanol, DMSO (dimethylsulfoxide), and acetonitrile to the renaturation buffer and further study using different vaccination methods, including antigen dose, times, adjuvant and more mice need to be elucidated.

Also, the results from in vitro studies and from studies with telomerase-deficient mutant mice need to be verified with genetically intact hosts.

Reversal learning requires behavioral flexibility: the mice need to adapt their previously learned behavior to stop pressing the old active lever and begin pressing the new active lever for food reward.

As described below, specific issues including some caveats associated with use of the CD4-cre TGF-βRIIf/f mice need to be addressed in revising the manuscript.

For instance: (a) Experiments of a sufficient number of mice need to be designed so that sensitivity and specificity of the approach can be quantified.

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The mice needed to take the drug in advance of a heart attack in order for it to be effective.

All tested mice needed to be fasted overnight (12 h) during the experiment to avoid the absorption of CUR being influenced by food.

Another important issue that one has to consider in the development of a vaccine for RA is that the results conducted on mice needs to translate to humans.

The role of Mc1r and agouti genes in effecting such pigment switching or modulation in spiny mice needs to be studied in detail.

Whether the presence of Leu226 in the HA of these H9N2 viruses is associated with host adaptation in mice needs to be investigated further.

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