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Mice were obtained from a colony of Swiss Webster mice maintained at the Centers for Disease Control and Prevention, Division of Vector-Borne Diseases (CDC/DVBD), Fort Collins, Colorado, USA, an Association for Assessment and Accreditation of Laboratory Animal Care AAALACC) accredited facility.
(b) GPR120 mRNA expression in BAT and iWAT from adult mice maintained at thermoneutrality (29 °C) or exposed to cold (4 °C) for 24 h, 7 days and 21 days (n=5) in the left, representative immunoblot of three independent assays of the relative changes of GPR120 protein, in the right.
(A) The wildtype mice maintained at room temperature were treated with norepinephrine (NE) or vehicle control.
The threshold for the selective loss of FAD fluorescence appeared ≤10% O2 in control mice (maintained at 37.0°C).
We observed that treatment with norepinephrine (NE) efficiently increased the expression levels of NGF in iWAT of the wildtype mice maintained at room temperature (Fig. 6A).
The volume fluorescence-imaging revealed no significant alteration of the tissue size and the general anatomy of sympathetic innervations (Fig. S3A), or density of the sympathetic arborizations (Fig. 5E and 5F), of iWAT of the 1-NaPP1-treated vs. vehicle-treated TrkAF592A/F592A mice maintained at room temperature for up to 1 week.
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Since our Cacna1fnob2 animals perform nearly as well as wild type controls, it is clear that in spite of the histological and electroretinographic evidence of severe retinal dysfunction, the Cacna1fnob2 mouse maintains at least some nearly normal visual processing in proximal levels of the retina.
As expected, urinary NE decreased with age in WT mice, while GHS-R ablated mice maintained NE at a level comparable to that of young mice (Fig. 5A).
In contrast, the Sgce-null and Sgca-null mice maintained normal parameters at this age.
C57BL/6J background wild type mice were maintained at the mouse facility of the Institute of Molecular and Cell Biology, Tartu University.
As shown in Figure 1, saline-injected mice maintained stable hearing response at any frequency tested during treatment.
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