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Adipose tissues (BAT, ScW, GnW), skeletal muscle and serum were collected from 12-week-old bmp8b WT and TG mice kept at 21 °C or from 5-week-old bmp8b WT and TG mice born and housed at 28 °C.
In contrast to the control group (normoxic mice kept at 20% O2) at low doses of 1 µg / kg body weight, mCPP triggers plasma protein extravasation (PPE) in the dura mater in hypoxic mice.
Female redbacks have an average of around 0.08 0.10 mg of venom, and experiments indicate that the median lethal dose (LD50) for mice at room temperature is 10 20% of this quantity (0.27 0.91 mg/kg based on the mass of the mice used), but that it is considerably more deadly for mice kept at lower or higher temperatures.
Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality.
Finally, the inhibition of COX activity not only attenuated diet-induced UCP1 expression in iWAT, but also increased weight gain in Sv129 mice kept at thermoneutrality.
Similarly, all of our studies were conducted using individually housed mice kept at the same 22°C environmental temperature, but it is well-known that temperature can significantly impact EE in mice [22], [23].
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The results indicate that AEF produced significant reduction of blood glucose levels 4 h after oral administration in mice kept fasting overnight at doses of 750 and 500 mg/kg.
Likewise, genetic deletion of UCP1 in mice causes weight gain when mice are kept at thermoneutrality [53].
The expression of TGF-β in Treg-depleted mice kept in a similar level at day 7, 28 and 56 (Fig. 7B).
Significant differences in fat tolerance were detected when mice were kept at 4 C. Hadh+/+ mice show only a moderate increase in the concentration of plasma triglycerides and fatty acids in comparison with the levels detected under conditions of 20 C. This difference likely indicates that hadh−/− mice might not be able to accelerate triglyceride clearance.
Brains of mice, kept under DD conditions, were removed at indicated circadian times, then immediately frozen by isopentane, and stored at −80°C until use.
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