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Consistent with these findings, 78% of mice inoculated by the ABL protocol with CT deletion strain P4 remained colonized to 48 hr after low dose infection, but none of the mice inoculated with multitoxin deletion strain KFV101 (P4ΔhlyAΔremainedA) remained colonized (Fig. 3A and 3D, Mann-Whitney, p<0.001, χ2 = 0.0006)).
c - From 450-600dpi 2 of 4 mice inoculated by the i.v. route had detectable PrPres amyloid in brain.
At later times, from 450-600 dpi, a few mice inoculated by the i.v. route had detectable PrPres amyloid in brain [ 26].
These studies characterized the molecular similarities of the agents causing BSE and vCJD and determined the lesion profile and incubation period patterns of different panels of mice inoculated by the two agents.
In mice inoculated by CellsRas-G12V+TNFαCMRas-G12V+TNFα CellsRas-G12V+TNFαCMRas-G12V+TNFα CellsRas-G12V+TNFαCMRas-G12V+TNFαr, and the percentage of mice with lagmetastases was higher (83%) comperiodto all other Cell-CM combinations (12-36%; Figuntil6C).
Mice inoculated by either IN or SL administration developed protective IgG antibodies against tetanus toxin challenge.
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By the next experimental setup, we compared the metastatic burden (metastasogenesis) in the lung and liver of mice inoculated i/v by VMR cells overexpressing RANTES vs empty vector at fixed time-points.
Three mice inoculated with GFP/Ap by intraperitoneal injection became infected as demonstrated by the appearance of morulae in a peripheral blood neutrophil and re-isolation of the bacteria in culture.
Again, a significantly higher concentration of IFN-γ was produced from splenic T cells obtained from mice inoculated with BCG-dHCM by all stimulators than those from mice uninfected or infected with BCG-261H.
In addition, 7 of 8 mice inoculated with 10 TCID50 or 10 TCID50 by the oculo-oronasal route seroconverted; 4 mice inoculated with 10 TCID50 by the intraperitoneal route showed no detectable antibody response to the virus (Table).
Therapeutic treatment: the same treatments were administrated in Groups A, B, C, and D, formed by 20 mice in each group, while the Group E was formed by 16 mice inoculated with PBS, as control.
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