With further development the difference in length of the circumference between Slc26a4+/− and Slc26a4−/− mice grew from a factor of 1.1 to a factor of 2.3 at E16.5 and 2.7 at E18.5 (Fig. 6B).
With the help of his cloning expertise, they hoped to create a chimera, a mouse grown from stress-altered cells injected into a host embryo — a spectacular show of the cells' developmental potency.
These data definitively demonstrate that pathologic new vessels in the Vldlr-/ mouse grow from the inner retina towards the avascular zone in the ONL and the RPE and not in the reverse direction.
A172 bacteria were isolated from a vaccinated mouse, grown for 15 generations under stress conditions (10 µg/ml novobiocin) [15] and used to establish 400 independent bacterial cultures, which yielded a total number of about 4×1011 bacteria (109, the number of cells/culture×400, the number of cultures).
Spirochetes were isolated from chronically infected mice, grown to late-log phase in BSK-H complete medium, harvested by centrifugation and analyzed for DbpA and DbpB synthesis by immunoblot analysis probed with a mixture of a FlaB mAb and anti-DbpA or -DbpB sera as described above.
MEFs were generated from second (G2) and fifth (G5) generation TERT-null mice grown to senescence.
Mouse VSMCs were grown from explants of mouse aorta removed from either MMP knockout or wild-type mice or C57BL/6J controls, as described previously.
The mice grew teratomas, a kind of tumor that grows from germline cells and which contains many types of tissues.
Anura Rambukkana of the MRC Centre for Regenerative Medicine at the University of Edinburgh and his colleagues isolated Schwann cells from adult mice, grew them in Petri dishes and infected them with M. leprae.
Both Dr Smith's group, working with neural stem cells from mouse fetuses, and Dr Terada's team, using bone marrow cells from adult mice, grew their harvest alongside embryonic stem cells in the laboratory.
