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As control for the specificity of the QC immunohistochemical labelling, QC knock-out mice generated on the basis of classical homologous recombination approach were included [ 25, 36].
Parp-1-KO mice generated on an SV129 background were backcrossed with CD-1 mice to obtain F5 CD-1 (∼97%)/SV-129 (∼3%) mice.
Moreover, again using the chronic catecholamine infusion model, we observed exaggerated contractile dysfunction, ventricular dilation and fibrosis in conditional I-1c expressing mice generated on an I-1 KO background.
HBM is observed in doubleridege mice having a spontaneous mutation leading to reduced DKK1 expression, Dkk1 heterozygous mutant mice and Dkk1 homozygous KO mice generated on a Wnt3 heterozygous mutant background.
Tg197 mice generated on CBA × C57BL/6 genetic backgrounds and littermate controls were bred and maintained at the animal facilities of the Biomedical Sciences Research Center, Alexander Fleming, Athens, Greece, under specific-pathogen-free conditions.
Homozygous IL10−/− mice generated on a 129 Sv/Ev genetic background, and 129 Sv/Ev controls were housed under specific pathogen-free conditions until weaning (3 weeks), when they were moved to conventional animal housing.
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Experimental cohorts of mice are generated on-demand and ready-to-use.
Taken together, the data describe the first genuine mouse iPS cell lines generated on human feeder cells without exogenous LIF, providing a reliable tool for understanding the molecular mechanisms of nuclear reprogramming.
Thus, the study, for the first time, reports mouse iPS cell lines generated on human feeder cells, providing a reliable tool for further dissecting the molecular mechanisms of nuclear reprogramming.
In summary, this study has demonstrated that H. trogontum infection in IL-10−/− mice generates acute-on-chronic typhlocolitis, thus reconfirming that infection of IL-10−/− mice with H. trogontum produces a picture similar to that observed in IBD, particularly CD.
The ERαflox/flox mice are inbred C57BL/6 mice, and the Col2α1-Cre mice were generated on a B6SJLF1 background and backcrossed more than six times with C57BL/6 mice.
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