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Unexpectedly, breeding BcorFl/+ mice to ß-actin-Cre mice generated apparently normal female (BcorΔ3/+) and male (BcorΔ3/Y) mice.
Knockout of either ID1 or ID3 alone in mice generated apparently normal phenotypes [ 31].
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Now, a Japanese team has figured out how to overcome nature's obstacles and has generated apparently normal mice by combining the genomes of two mouse eggs.
Moreover, the chimeric mice generated from CiPSCs were 100% viable and apparently healthy, unlike those generated from retrovirus-induced iPS cells.
Additionally, the highly symmetric shape of reconstructed cell lineage trees in these mice, generated by phylogenetic inference using mutations accumulating in single cells, similarly supports the apparently stochastic nature of lineage differentiation occurring during embryogenesis.
To visually examine beige adipocytes, we also utilized the beige-chaser mice generated by crossing Ucp1-Cre with Rosa26mT/mG mice.
But instead of forming scar tissue, the mice generated new cells that were indistinguishable from neighboring healthy heart cells.
Subsequent breeding of heterozygous OGR1fl/+ mice generated homozygous OGR1fl/fl mice.
Briefly, CD146floxed/floxed mice crossed with Tek+/Cre mice generate Tek+/CreCD146floxed/+ mice.
These mice were maintained on a C57Bl/6N background (>8 crosses), and experimental mice were generated by crossing TK+ mice with 129Svev WT mice.
CD4Cre Prdmice/fl mice were generated by crossing Prdm1fl/fl mice with CD4Cre mice.
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