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Subsequent mouse husbandry and experimental treatment followed the description above and the conditioning protocol used for Experiment 2. Mice from the BALB and B6 strains were tested (N= 16 mice from three to four litters per strain).
Brains from male C57BL/6 mice from three different age groups (3, 10, and 30 months of age) were sectioned coronally through the dorsal hippocampus and horizontally through the intermediate hippocampus.
For in vivo imaging, we used mice from three different TG mouse lines and their strain-matched wild-type (WT) control animals.
These "moth-eaten" fibers were not artifacts because they were reproducible in multiple mice from three CAG200 lines.
Totally 29 mice from three litters were used for rapamycin treatment (n = 15) and control (n = 14).
Cohort 2 consisted of 72 male mice from three CRL breeding facilities (North Carolina (NC), Michigan (MI) and New York (NY)).
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Representative dot plots from n = 9 (Balb/c) or n = 15 (B6) mice from two independent experiments.
Primary human prostate xenografts were established in athymic nu/nu mice from eight of eight benign and five of five prostate cancer tissues, collected from a total of 10 patients who underwent radical prostatectomy for the treatment of prostate cancer.
Mice from four replicate High Runner (HR) lines have been selectively bred for voluntary running on wheels, and run approximately three times as many revolutions per day as do mice from four non-selected Control (C) lines.
Interestingly, when Chmp7 expression was examined in the spinal cord of presymptomatic mice from two separate mouse models of MND, the SOD1-G93A model mofel of Amyotrophic Lateral Sclerosis (ALS) and the SETX-R2136H mofel of ALS4, an adolescent-onset form of ALS, we found no significant differences between WT and mutant mice (Supplementary Information, Fig. S5).
In the current contribution we studied in deeper form the disruption of motor performance produced by the intraperitoneal administration of 3TB in mice from two strains (CD1 and C57BL6).
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