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By growing mice from these cells, researchers can gain insight into the role that the missing genes play in health and disease.
Importantly, we obtained live chimeric mice from these iPS cells with high degree of chimerism.
To realize the utility of these resources, efficient and accessible methods of generating mutant mice from these ES cells are necessary.
Furthermore, the F1 Sirt1+/− mice from these crosses were fertile, and had a general phenotype indistinguishable from that of Sirt1+/− or Sirt1+/+ mice.
We were unable to statistically compare tumor burden in mice from these groups at the same time point, as mice with shHIF bone metastases lived longer than the parental or shNT controls.
The finding that B6 freezing was sensitive to conspecific distress, but BALB freezing was not, is remarkable because mice from these strains also differ in adolescent social motivation [27], [28].
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Male F1 mice without any obvious phenotype were mated with wild type mice, female mice derived from these matings (G2) were mated with the F1 father.
Although expression of these genes is upregulated in P4 Chrnb2−/−micent mice, retinas from these mice are phenotypically normal (Figure 2C, 5C and D).
Although it was not our goal to generate a mouse for every different FP tested, one agouti mouse from these matings that exhibited bright red fluorescence (Fig. 5E,F) was used to establish a ROSA26 Cherry mouse line.
C57BL/6J mice from which these mice were derived were used as control mice.
In contrast, administration of AF or immunization with GP42-GFP did not protect the mice from challenge; these mice rapidly lost 25% of the starting body weight and were subsequently euthanized.
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