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Male mice from seven strains (Balb/cOla, C57BL/6JOla, C57BL/6N, DBA/2Ola, DBA/2JIco, FVB/N, NMRI) - referred to as Balb/c, BL/6J, BL/6N, D2Ola, D2JIco, FVB and NMRI respectively - were purchased from Harlan-Winkelmann GmbH (Borchen, Germany; Balb/c, BL/6J, D2Ola, FVB and NMRI) and Charles River GmbH (Sulzfeld, Germany; BL/6N and D2JIco).
For these baseline environmental preference measurements, juvenile mice from the A, BALB, B6 and DBA strains were examined (N= 32 mice from seven to eight litters per strain).
For these measurements of social preference, juvenile mice from the A, BALB, B6 and DBA strains were tested (N= 32 36 mice from seven to nine litters per strain).
To control for the potential effects of presenting a novel set of environmental cues in the testing arena, the preferences of a third group of mice (N= 32 mice from seven litters) that received 24-h social housing sessions in the aspen or paper environment alternated with social housing in the newspaper environment were assessed.
A detailed comparative analysis of female 8- to 10-week-old mice from seven different inbred strains (A/J, AKR/J, Balb/cJ, C57BL/6J, DBA/2J, C3H/HeJ and 129X1/SvJwT), for example, revealed that the different mice showed an overall variation in regard to ALT, liver weight and liver fibrosis when fed an MCD diet [ 83].
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Subsequent mouse husbandry and experimental treatment followed the description above and the conditioning protocol used for Experiment 2. Mice from the BALB and B6 strains were tested (N= 16 mice from three to four litters per strain).
Representative dot plots from n = 9 (Balb/c) or n = 15 (B6) mice from two independent experiments.
Primary human prostate xenografts were established in athymic nu/nu mice from eight of eight benign and five of five prostate cancer tissues, collected from a total of 10 patients who underwent radical prostatectomy for the treatment of prostate cancer.
Mice from four replicate High Runner (HR) lines have been selectively bred for voluntary running on wheels, and run approximately three times as many revolutions per day as do mice from four non-selected Control (C) lines.
Interestingly, when Chmp7 expression was examined in the spinal cord of presymptomatic mice from two separate mouse models of MND, the SOD1-G93A model mofel of Amyotrophic Lateral Sclerosis (ALS) and the SETX-R2136H mofel of ALS4, an adolescent-onset form of ALS, we found no significant differences between WT and mutant mice (Supplementary Information, Fig. S5).
Brains from male C57BL/6 mice from three different age groups (3, 10, and 30 months of age) were sectioned coronally through the dorsal hippocampus and horizontally through the intermediate hippocampus.
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