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Mice from four replicate High Runner (HR) lines have been selectively bred for voluntary running on wheels, and run approximately three times as many revolutions per day as do mice from four non-selected Control (C) lines.
To increase precision and accuracy of estimates, we increased the number of mice from four per data point as recommended by ASTM (1984) to 5 10 mice.
To rule out the possibility of bacteriological infection of the gastrointestinal tract, which could affect the colon mucosa, the feces from mice from four randomly chosen animal cages were collected and screened for Helicobacter bilis, H. hepaticus, H. rodentium, H. muridarum and H. typhlonius by PCR (Surrey Diagnostics, UK).
In the present study we examined differential gene expression profiles in lungs of adult mice from four treatment groups [ETS-, sandne-, and OVA-exposed (ESO); HEPA-filtered air-, S-, and O-exposed (ASO); E- and O-exposed, then O-exposed (EOO); A- and O-exposed, then O-exposed (AOO); Figure 1] using extracted mRNA.
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Subsequent mouse husbandry and experimental treatment followed the description above and the conditioning protocol used for Experiment 2. Mice from the BALB and B6 strains were tested (N= 16 mice from three to four litters per strain).
For in vivo imaging, we used mice from three different TG mouse lines and their strain-matched wild-type (WT) control animals.
Primary human prostate xenografts were established in athymic nu/nu mice from eight of eight benign and five of five prostate cancer tissues, collected from a total of 10 patients who underwent radical prostatectomy for the treatment of prostate cancer.
Brains from male C57BL/6 mice from three different age groups (3, 10, and 30 months of age) were sectioned coronally through the dorsal hippocampus and horizontally through the intermediate hippocampus.
In the current contribution we studied in deeper form the disruption of motor performance produced by the intraperitoneal administration of 3TB in mice from two strains (CD1 and C57BL6).
Each group had three mice from two independent experiments.
Thus, no therapeutic effects of lithium were demonstrated in female SOD1G93A mice from two strain backgrounds in the Bendotti studies.
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