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Five mice from each group were euthanized at 3 d.p.i.
Subsets of mice from each group (n=10) were weighed and euthanized.
Five mice from each group were euthanized by cervical dislocation at fasting state.
After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein.
Thereafter, four mice from each group were killed weekly for 8 weeks, and their aortae were frozen for immunohistochemical analysis.
By the end of the treatment, five mice from each group were euthanized by cervical dislocation at fasting state.
Twenty-four hours after surgery, mice from each group were deeply anesthetized with Equitensin (120 μl/mouse i.p).
At the end of treatment, three mice from each group were euthanized by cervical dislocation at fasting state.
Uninfected mice from each group served as controls.
Five mice from each group were sacrificed at specific time points.
Equal amounts of TIF from spinal cords of five mice from each group were pooled.
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