We prepared two independent groups of mice for behavioral tests.
Of course, it might be equally possible that the originally reported minor pattern completion deficit in CA3-NR1 knockout mice may have resulted from behavioral variations during those particular sets of experiments (it is noted that an unusually large group of control mice were used in comparison with a much smaller group of mutant mice).
Establishing the role, this study covers a wide territory from behavioral analysis of mouse behavior to alterations in signaling reactions and biochemical studies of protein complexes.
Comparing four different mouse transgenic lines overexpressing different regions of human chromosome 21, only mice from line 152F7 revealed behavioral and morphological abnormalities, which were attributed to Dyrk1A [40].
Moreover, studies of memory impairment in a mouse model of AD have found that the deletion of PrPc expression dissociated Aβ accumulation from behavioral impairment in mice, suggesting that the cognitive deficit normally resulting from some aspect of the Aβ42 cascade selectively requires PrPc [ 39].
Importantly, circadian deficiencies observed in this 384 well based siRNA knockdown mimic results from siRNA based knockdown in 35 mm culture dishes, fibroblasts from the respective knockout mice, and behavioral rhythms in mice-carrying loss-of-function alleles of the respective genes [27], [29].
Thus, optogenetic or chemical manipulations to re-active a transcriptionally pre-activated subset of neurons were found to be sufficient to trigger, or to recapitulate at least in part, the memory recall process, as judged from mouse behavioral criteria.
Analogously to worms, mice that harbor only one Igf1r copy are protected from behavioral and pathological impairments that stem from the aggregation of human Aβ in the brain.
One approach to investigate whether and how personality traits are associated with the level of spontaneous physical activity is to study homologous behavioral traits in mice from lines that have been selectively bred for high voluntary wheel-running behavior (Swallow et al. 1998; Garland Jr 2001) and compare these to non-selected control mice.
