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Pathological evaluation was limited because tissues from a number of mice found dead were not suitable for histological fixation.
Moribund mice were euthanized and along with mice found dead between observation periods, the carcasses were frozen at minus 20°C, later thawed, spleens were resected, homogenized in 1 mL 1% protease peptone, serially diluted and 10 µl of the homogenate was drip-plated on TSA and LB (with or without 0.015 mg gentamicin/mL) agar plates.
A subset of miR-1 null mice found dead at birth had ventricular septal defects (arrows).
During the survival study, mice were observed twice daily, at 7 am and 6 pm; any mice found dead were removed from the appropriate cages.
Date of death was noted for mice found dead, and mice found to be so ill that they were expected to die within the next 24 48 h were euthanized, with the date of euthanasia taken as the date of death for life table calculations.
Mice found dead were assigned a score of 15.
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B. Single MuRF2+/+ heart from mouse found dead 21 weeks high fat diet reveals amorphous way infiltration (arrows) and rare leukocytes infiltrations.
Consistent with the reported E12.5 lethal phenotype of Angpt1 null mice (Suri et al., 1996 ; Jeansson et al., 2011 ), Angpt1 −/− mice were found dead at E12.5.
Brains were collected for histological analysis but unfortunately not every brain was suitable for histological analysis, as some of the mice were found dead unexpectedly in the beginning of the study.
Starting in early June 2012, increased numbers of mice were found dead in the barn.
In addition to mice that were found dead, mice with a weight loss of more than 25% of the starting bodyweight were euthanized and recorded as dead.
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