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Development of "Mouse Avatars" entails implantation of patient tumor samples in mice for subsequent use in drug efficacy studies.
Indeed, transfer of Ag-pulsed, bone marrow-derived mDCs to the airways of naïve animals is sufficient to sensitize mice for subsequent development of allergic asthma [40] [43].
I was able to establish a transgenic line from one of these founders (F66), and I backcrossed this line extensively to C57 BL/6 mice for subsequent analysis.
To circumvent the limitations of genetic variability, we attempted numerous times to recapitulate our IUP analyses with a C57/B6 inbred strain but failed, simply due to the difficulty of obtaining enough 2M and 2F male mice for subsequent metabolic analysis.
Since Ppp2cα fl/fl and AlbCre + mice displayed similar level of PP2Ac protein and phosphatase activity in livers, we used Ppp2cα fl/fl mice for subsequent studies.
Conceivably, the instrumentation and procedures involved could be optimized and/or validated in a minimally invasive, behavior non-modifying format to identify individual sighted mice for subsequent behavioral experimentation.
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As previously reported, EGR-2 deficient mice are perinatally lethal [15], therefore, in order to analyze the role of EGR-2 in T cell responses in vivo, we crossed EGR-2flox/flox mice to CD4-Cre mice (mice used for subsequent studies reported in this paper were backcrossed onto a B6 background for at least 10 generations).
The GFAP/Gp120+/+//Faah−/− mice were then crossed for two more generations (F3 and F4) to obtain the colonies of GFAP/Gp120//Faah−/− mice utilized for subsequent experiments, (see Supporting Information Figure S1 in supplemental methods for genotyping of these mice).
All mice used for subsequent experimental work were killed by cervical dislocation [Schedule 1: UK Animal (Scientific Procedures) Act 1986].
The increased availability of SP cells from the mouse mammary epithelium encouraged us to move to a mouse model for subsequent studies.
Each treatment group consisted of 4 5 mice for every subsequent time point.
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