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This study indicates that worsened outcome from MCAO of apoE-deficient mice is not attributable to any detectable vascular effects and offers validity to use of apoE-deficient mice for study of apoE as a factor in cerebral ischemic pathophysiology.
However, the resulting double homozygotes bred successfully, generating sufficient apoE−/−TG2−/− mice for study.
However, mutant mice are fertile, and breeding heterozygous male and female mice is the standard approach for producing heterozygous mice for study (Donehower et al., 1992).
TV-G co-directed the design and implementation of the study, obtained cohorts of mice for study, performed 3 D culture experiments, interpreted data, and drafted the manuscript.
Alternatively, the regulation of gene expression in fibrotic glomeruli could be a manifestation (rather than a cause) of fibrogenesis, though we attempted to minimize this possibility by selecting mice for study at an age prior to the appearance of glomerulosclerosis.
The RARα1-/+ female mice were crossed with hemizygous male MMTV- wnt1 mice followed by intercrossing of F1 progeny that was RARα1+/- and MMTV- wnt1 transgenic, until sufficient mice for study were obtained.
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Douam F, Ploss A. The use of humanized mice for studies of viral pathogenesis and immunity.
Douam, F, and A Ploss. "The use of humanized mice for studies of viral pathogenesis and immunity".
However, insufficient development and function of human natural killer (NK) cells and T cell subsets limit the applicability of humanized mice for studying cancer biology and therapy.
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