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Catheters were implanted into a carotid artery and a jugular vein of mice for sampling and infusions respectively 5 days before the study [ 24].
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For mice samples, DNA samples extracted from blood of uninfected mice were used as negative controls.
Eight days after treatment, blood was collected and pooled from two mice for each sample.
The mean number of cycle thresholds (Ct value) for mice sampled at day 3 PI was 32.47 ± 2.11; at day 6 PI, the Ct value was of 35.52 ± 2.01 (p = 0.001).
Before the sacrifice, mice were sampled for blood.
Heart rate and activity for individual mice was sampled continuously in 2-s bins at 30-s intervals and recorded against a date/time stamp using the dataquest A.R.T.™ software (Data Sciences International).
Immediately after sampling and removing the corpse, surface soil was sampled from under the mouse for a total of four samples associated with each decomposing mouse corpse (or five samples total for mice for which two abdominal samples were taken).
For sampling, mice were deeply anesthetized with pentobarbital and transcardially perfused with ice-cold phosphate-buffered saline (PBS) containing 4% paraformaldehyde (PFA).
A similar pattern was observed in mouse for a sample of 542 different-strand overlaps with ~54% convergent, ~37% divergent, and ~9% embedded forms.
> -wrap-foot> Varees are means ± SEM for n = 10 mice sampled per group.
After 24h, mice were sacrificed for sample collection.
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