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Mice were backcrossed to C57BL/6J mice for one additional generation.
Implantation in subcutaneous pockets in nude mice for one week demonstrated that vessel formation was significantly higher in the VEGF sustained-release group compared to the fast release group.
USC that were induced to differentiate also expressed UC markers (Uroplakin-III and AE1/AE3) or SMC markers (α-SM actin, desmin, and myosin) after implantation into athymic mice for one month, and the resulting tissues were similar to those formed when UC and SMC derived from native ureter were used.
Melzer notes that we used transgenic mice for one end point and normal mice for others.
Figure 4C shows all the pairwise interactions between the mice for one of the groups.
Mice harboring the floxed Rc3h1 allele (Rc3h1 lox/+) were then crossed to Rosa26 Cre knock-in mice for one generation.
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Either the mouse IL-27 recombinant protein (rmIL-27; 1 μg per mouse for seven days) or anti-mouse IL-27 p28 functional grade purified antibody (200 μg per mouse for one day; R&D System, Minneapolis MN) was injected hypodermically.
Analysis of a knock-in mouse for one of the reported mutations (N641Y) showed homozygous mice to be significantly more susceptible to seizures [ Singh et al, 2009], supporting the hypothesis that this mutation is disease-causing.
Mice from the TauP301L responder line were crossed with mice from the BAC-LRRK2 mouse line for one generation to obtain LRRK2.TauP301L responder mice on an FVB background.
Therefore, whereas in mice lacking expression of both IL-2 and IL-15 or both of their receptors substantially reduced numbers of Treg were consistently found in the thymus, in mice deficient for one or the other cytokine or receptor discordant results were obtained.
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