Sporozoites were collected from slurry using a renografin gradient (Bracco Diagnostics), and suspended in either M199 media containing 3% naïve mouse serum for injection into BALB/c mice or in lysis solution (RNAqueous Micro kit, Ambion) for RNA extraction.
(*, P < 0.05 versus WT, n = 9 mice for melanoma injection, n = 10 mice for fibrosarcoma injection in each group).
E3.5 blastocysts were collected from ICR mice for hESC injection.
Handling of the mice for subcutaneous injection caused immediate arousal from the torpid state, but the rate of arousal following β3 antagonist administration was blunted (70% of the rate following vehicle administration) (Fig. 4).
Use 0.2 mL per mice for intraperitoneal injection.
During the habituation period two mice died (one found dead and one was culled for hydrocephalus) in the standard housed group, so that this group consisted of 18 mice for tumor injection.
For IP injection of His-NLS-Cre, 100 µl of a 1.84 µg/µl stock was injected per adult mouse; for intraocular injection 2 µl of a 3.52 µg/µl stock was injected per eye at P5 using a 30 gauge needle.
For IV injection into the tail vein, 400 µl of an Ad-Cre stock (3×109 plaque forming units/ml; Virenz, Baltimore, MD) was injected per adult mouse; for intraocular injection, 1.5 µl of the same virus stock was injected per eye at >P21 with a glass micro-needle.
Similarly, another group of male mice were used for injection of 0.2 ml saline on day 1, or 1.0 or 2.0 or 6.0 mg/kg amphetamine on day 2. For testing amphetamine-induced spontaneous USVs, male and female mice were tested independently at doses of 0.1, 0.2, 0.5, 2.0, and 6.0 mg/kg.
