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Next, we conducted gene delivery of the adenoviral vectors to BALB/c mice for further exploring the effects of Emuc in vivo.
We generally avoided using TLG 498+ Cre 3487+ transgenic mice for further breeding, as germline recombination sometimes occurred resulting in broad EGFP-F expression in the next generation.
No adverse side effects on general health were observed during the 2 week colonization period, after which serum was collected from the sacrificed mice for further investigation.
Thirty days after injection, tumors were excised from tested mice for further analysis.
Next, we selected some candidate miRNAs highly expressed in sera and cancer tissues of DCKO mice for further evaluation.
The SB transposon also acts as a molecular tag to, without additional breeding, efficiently identify mutated genes and prioritize mutant mice for further characterization.
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In this study we chose human and mouse for further analysis.
In conclusion, these findings in lupus-prone mice call for further investigation of the role of IL-23 in SLE.
All BM-transplanted eNOS−/− mice selected for further study showed >85% chimerism at 4 months after transplantation.
However, very low levels of bile salt and urate transport are observed with MRP4, making generation of Mrp4 knockout mice necessary for further analysis of their transport.
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