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Mice were sacrificed 24 hours after treatment, with 3 mice for each treatment and 3 independent experiments performed.
The results were expressed as %ID/g with a minimum of 3 mice for each condition.
The mice were scarified at 20 min, 1 h, 4 h, and 12 h (five mice for each group) after injection.
To determine the in vivo GRPR-targeting efficacy of labeled peptides, biodistribution of female Balb/c mice were realized with a minimum of 4 mice for each condition.
There were four groups of mice (control, MW, chitosan/Fe3O4@IL + MW, chitosan/Fe3O4@IL) with five mice for each group.
Mammary glands were analyzed from two individual mice for each genotype.
Groups of 16 mice for each treatment (FK866 vs. DMSO) were used.
We have used a total of 79 mice; for each animal, one retina was analyzed.
CD11b-DTR mice (5 mice for each group) were given intraperitoneally (i.p).
Each experiment was repeated thrice, with a total of 20 mice for each experiment.
At least 3 mice for each experimental group were used for quantitative analysis.
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