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We tested juvenile (5 6 weeks) and adult (10 11 weeks) db/db mice for behavioral depression in forced swim test (FST), psychosis-like symptoms using pre-pulse inhibition (PPI) test, anxiety behavior employing elevated plus maze (EPM) test, locomotor behavior and thigmotaxis using open field test and working memory deficits in Y-maze test.
We prepared two independent groups of mice for behavioral tests.
We used Sema5A null mice for behavioral testing, including the three-chambered apparatus, which provides a simple social approach task.
SHIRPA (SmithKline Beecham, Harwell, Imperial College, Royal London Hospital, phenotype assessment) was used as a primary screen to assess 1.5-year-old CAST mice for behavioral alterations compared with the non-transgenic littermate controls.
Statistical significance was evaluated using two-way repeated measures ANOVA and Tukey post-hoc comparisons (Sigma Stat 3.0.1, SPSS Inc .. Mice for behavioral testing were housed in groups of 2 to 4 in individually-ventilated cages, with food and water ad libitum and maintained on a 12 h light-dark cycle.
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We phenotyped almost 800 mice for 3 behavioral traits: behavioral despair (immobility in the TST), general anxiety (thigmotaxis in the Open Field), and general motor activity (distance traveled in the Open Field).
All mice sent for behavioral analysis were analyzed subsequently by ERG at 13 weeks post injection upon their return to the University of Florida (smCBA-mGC1: n = 3, hGRK1-mGC1: n = 2).
Male mice used for behavioral studies were >99% congenic, as assessed by single nucleotide polymorphism (SNP) analysis (Charles River).
Conceivably, the instrumentation and procedures involved could be optimized and/or validated in a minimally invasive, behavior non-modifying format to identify individual sighted mice for subsequent behavioral experimentation.
Another 12 mice were used for behavioral tests.
Eight to ten-week-old male C57BL/6J mice were used for behavioral experiments.
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