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Blood, bronchoalveolar lavage fluid spleen and lung tissue were excised from mice for analysis.
We sacrificed the mice for analysis at 13 to 14-weeks-old.
On day 8, lungs and lymph nodes were taken from mice for analysis (Figure 2).
Heterozygote mice were then crossed to yield homozygous double-knockout mice for analysis.
Primary macrophages were harvested from Jnk1+/+ or Jnk1−/− mice for analysis of alterations in gene expression as previously described [13].
Restimulation assays were performed with splenocytes from immunized and naïve mice for analysis of T cell responses.
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One mouse died shortly after inoculation, leaving 15 mice available for analysis.
Figure 6 illustrates the injection site locations for the eight WT and nine DKO mice used for analysis.
The mice used for analysis were backcrossed to C57Bl6 for a minimum of four generations.
The mice used for analysis of absolute cell counts and cytokine production were sacrificed at 24 h post surgery.
However, this did not reach significance due to the diminished numbers of peritoneal CD3 cells in wild-type mice available for analysis (data not included).
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