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Skin samples from 70% of mice fed upon by WT-infected nymphs were culture positive, while all skin samples from mice fed upon by the bba64 mutant-colonized nymphs were culture negative (Table 2).
Also, all skin cultures from mice fed upon by bba64 mutant-infected nymphs were negative for borrelial growth (Table 2).
Furthermore, 89% of skin samples from mice fed upon by the bba64 complemented mutant-infected nymphs were culture positive, demonstrating the restoration of the WT phenotype (Table 2).
Using an IFA, we determined whether SFTSV antibodies were present in serum samples from mice fed upon by nymphal and adult ticks.
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For study of nymph feeding transmission of SFTSV, 10 uninfected mice were fed upon by 10 nymphs molted from larvae that fed on SFTSV-infected mice until engorged.
Upon histological examination, the livers of mice fed with HFD exhibited steatosis.
Here we characterized the metabolic features of mice fed high-fat diets and investigated the impact of leptin upon the lipogenic program which was found to be suppressed by HFD feeding through a proteomics approach.
The rate of glucose clearance upon i.p. insulin injection was also lower in mice fed HFD, further suggesting insulin resistance (Figure 1D).
Upon stimulation with α-GalCer, mononuclear cells from the livers of C57BL/6 mice fed the PRD produced less IL-4 and IFN-γ than did those from mice fed the CD (Fig. 5d).
No mice fed CD or KD died.
n=5 (Tg mice fed 45% HFD), 3 (non-Tg mice fed 45 HFD).
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