These experiments, combined with his later research on mice, established that memory is centred on the synapses, as changes in synaptic function form different types of memory.
Experiments on wildtype mice established that the host supplied terminal Schwann cells (TSCs) at the NMJs of transplanted muscles.
In contrast, Prnp−/− Nagasaki mice were established on a pure C57BL/6 background [18] and found to be IMERV-competent.
p53 PZU (PZU), p53 PMZU (PMZU) and p53 PZS (PZS) mice were established on C57BL/6J and CBA mixed background and were backcrossed to C57BL/6J background for three generations.
IR flox/flox mice, initially generated from C57BL/6J and CD-1 background, had been backcrossed for at least five generations on a C57BL6 background, and Col2-Cre mice, initially established on a C57BL6/129sv background, had been backcrossed for 10 generations on a C57BL6 background before being intercrossed with IR flox/flox mice.
Previously, we successfully established one mouse ES cell line on human foreskin-derived fibroblast (HFF) cells, which could be passaged at least 30 times, retaining an ability to support ES cell pluripotency (Li, et al. unpublished data).
In the current study, researchers switched mice who were well established on a long-daily-fast regimen to a weekly pattern of five days on followed by two days off.
Optimal 5' and 3' RACE conditions were established on mouse C2C12 cells transfected with the DUX4c genomic clones to avoid the background generated by hundreds of homologous human DUX genes [2], [29], [33] [35] (see Supporting Information S1 and Supplemental Fig. S1).
Murine orthotopic HCC model was established on BALB/cAnN-nu athymic mice.
Hs766T and MIA Paca-2 subcutaneous tumors were established on the flanks of NCR-nu nude mice.
