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The thyroids were fixed in Clarke's fixative (75% V/V ethanol: 25% V/V glacial acetic acid) 18 h after perfusion of the mice, embedded in paraffin.
At 10 dpi DRG, sciatic nerve and plantar skin were dissected from transcardially perfused (PBS followed by 4% paraformaldehyde) mice embedded in cryostat medium and frozen.
Brain segments were obtained from control and TBI mice, embedded in tissue freezing medium (TBS, Fisher, Pittsburg, PA, USA), frozen, cut into 30-A-m-thick sections and placed on glass slides.
Briefly, tissue or embryos were excised from C57Bl/6 mice, embedded in tissue freezing media (TFM, Triangle Biomedical Sciences, Durham, NC) and sectioned at 16 μM on a Leica CM 3050 S Cryostat (Leica, Deerfield, IL).
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And Hella Jongerius, a Dutch designer, developed a line called "My Soft Office" that included a bed with a computer screen in its mattress and a keyboard and mouse embedded in a "smart pillow".
For immunohistochemical analysis, hindlimbs from normal mice and CIA-affected mice were embedded in CMC gel with hexane, and cryostat sections (5 μm) were generated.
Spinal cord and spleen were separated from naive mice and PBS-, shNC-MSC-, or sh Becn1-MSC-treated EAE mice, and embedded in OCT.
Brains from adult MeCP2−/y and wild-type mice were embedded in Tissue-Tek OCT compound (Sakura) and frozen in dry ice-cold isopentane.
Briefly, 4% paraformaldehyde (Sigma, USA) fixed tissue from transcardially PBS perfused mice were embedded in paraffin using an Excelsior tissue processor (Thermo, USA) and sectioned at 5 µm.
Finally, islets from 12-weeks old RIP1-Tag2 mice were embedded in collagen to ascertain whether they harbored angiogenic properties ex vivo.
Pancreata from wild-type and Pten+/− mice were embedded in paraffin.
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