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The CI was defined as the ratio of the experimental strain to the WT strain recovered from the mice divided by their ratio in the initial inoculum [20].
Perinatal lethal ratios were calculated as number of perinatal lethal ∼week-3 mice divided by total number of week-3 mice.
Relative susceptibility is defined as the ratio of the effective ID the low-dose inoculum delivered to the test mice divided by the ID it delivered to control animals.
bNumber of diseased mice divided by all mice.
cScore of diseased mice divided by all mice ± SEM. dTotal of days of onset divided by the number of diseased mice ± SD. * e P < 0.05, χ2 test.
Relative tumour growth inhibition was calculated by relative tumour growth of treated mice divided by relative tumour growth of control mice (T/C).
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Total oxygen consumption was calculated as accumulated oxygen uptake for each mouse divided by its body weight.
Analysis was performed by means of measurement of BDA+ axonal densities within the cortex of the ischemic hemisphere (six fields per section) from eight sections of each mouse divided by total mean densities of all mice as described elsewhere.
Mice were assigned to treatment groups by cage mates, or in cases where fewer than five per goup were used, mice were divided by randomly removing some mice and combining them in a new cage.
Heterozygous mice were divided by litter and gender so that litter mates and cage mates of the same gender were used for comparison at each time point.
Wt and ASMko mice were divided by gender in groups of ten animals each.
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