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The C3H mice developed very small, poorly progressive B16 tumors (not shown).
In conclusion, the resistance to ECM development of LTα and LTβR deficient mice was not attributable to decreased parasitaemia or thrombocytopenia, and the ECM resistant mice developed very high parasitaemia and died of severe anemia within 3 weeks.
By 9 days after gene deletion, these mice developed very obvious hepatomegaly that was associated with increased BrdU and Ki67 staining, and the mice had to be sacrificed.
In fact, the three u-PA-/ mice developed very mild arthritis, each with a maximum score of only 1. Another immune complex-driven arthritis model is the K/BxN serum transfer model, in which serum from K/BxN mice, which develop spontaneous arthritis, is able to transfer the disease to naïve mice [ 19].
Once again the three u-PA-/ mice developed very mild arthritis, with a maximum score of only 2. By histology, C57BL/6 mice show massive cellular infiltration, cartilage damage, proteoglycan loss, bone erosion and fibrin ogen) staining following K/BxN serum transfer.
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In the p19−/− mice immunized by the s.c. route, arthritis was significantly inhibited with only 40% of the mice developing very mild arthritis.
We and other workers have previously shown that u-PA-/ mice develop very mild CIA [ 14, 15], whereas these same mice develop more severe AIA [ 12] and methylated BSA/IL-1 arthritis [ 13] compared with C57BL/6 mice.
In the chronic systemic collagen-induced arthritis (CIA) model, however, we [ 14] and other workers [ 15] have found that u-PA was deleterious, with u-PA-/ mice developing very mild disease and little fibrin deposition.
This is in marked contrast to control mice where disease developed very rapidly, becoming moribund within three days following the onset of hyperglycemia.
Unfortunately, metastasis formation could not be studied on this model because mice developed tumours very rapidly and died.
C3H.Q μMT mice developed only very mild oedema for no longer than 2 days (Fig. 4b), leaving no histological changes (data not shown).
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