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The amount of food allotted each cage of mice designated for caloric restriction was determined based on (weekly calculated) ad libitum food consumption for entire cages of gender-, genotype-, and birth date-matched controls; these values were averaged over the number of cages within each such group.
Mice designated for transmission electron microscopy (TEM) analysis were anesthetized with an ip injection of 0.1 mL 12% pentobarbital containing 1 IU heparin.
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For these control mice, designated Nf1 GRDCTL/+, we utilized the identical targeting strategy but arginine 1276 was left intact.
We determined that these conditional knockout mice, designated Gjb2-CKO, have a severe hearing loss.
From these, two lines of activin transgene-integrated mice (designated ACM3 and ACM4) and one line of follistatin transgene-integrated mice (designated FSM) were obtained.
The two lines were crossed, resulting in ESR-Cre/BDNFfl/fl mice (designated as BDNFind−/− mice).
All male inbred BALB/c mice which were designated for this study (n = 20) were purchased from the Institute of Medical Research, Kuala Lumpur, Malaysia, at around five weeks of age just after weaning.
The anatomy resources designated for this study were the Adult Mouse Anatomy (MA) ontology and the set of anatomy concepts contained in the NCI Thesaurus (NCIt).
They exclude weapons designated for decommissioning.
Both players were designated for assignment last week.
Meanwhile, infielder Josh Satin was designated for assignment.
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CEO of Professional Science Editing for Scientists @ prosciediting.com