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(H) Chimeric mice derived from injecting C57/B6 8 cell embryos with NPG-ESCs.
Wt and ob/ob mice derived MPs were cultured in the presence of DMEM+10% FBS.
Both sdy−/− mice and wild-type mice derived from heterozygote crossings were used in all experiments.
After genotyping of the pups, Pkd1flox/-: Ksp-Cre mice derived from these crosses were used as experimental animals.
The tumors in the mice derived from the cells were observed at ∼10 days after the cell inoculation.
In mice derived from line 37, lung lesions were not visible by MRI until about 28 32 weeks on dox (Figure 6A).
Genotyping of 310 mice derived from intercrossing Mariusz heterozygotes identified 7 informative recombinants that narrowed the mutation-containing region to a maximum of 2.5 Mb (Figure 3A).
This BM transplantation led to more than 95% of blood cells of recipient mice derived from the donor BM cells 2 months later (result not shown).
In LH mice derived from Founder #24, lethal T-ALL develops after a median 210 days with a penetrance of 60% (Fig. 2A).
Additionally, the mice derived on a Rest+/− background showed no discernable phenotypic changes as compared to their wild-type litter-mates [6].
To examine the role of established tumor murine models we first used WT and KO mice derived from WT (S1)×KO (S4) crossmating.
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