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The vaccinated mice demonstrated efficient clearance of the virus after challenge and resistance to lethal infection.
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Studies in mice have demonstrated efficient induction of CD8+ T cell responses by NKT lymphocytes through early IFN-γ production during acute virus infection [33], [38], [78].
Crosses using Cdx2-FlpO transgenic mice demonstrated the efficient recombination of multiple Flp recombinase-dependent reporters in the lumbosacral spinal cord.
The same group later demonstrated efficient PrPC knockdown in mice expressing an shRNA transgene, although they did not report whether these mice were protected from prion disease [25].
We previously demonstrated efficient generation of transgenic mice by the method of injection of ES cells into 4 8-cell embryos (Huang et al., 2008).
We have also engineered a compact self-regulated AAV which demonstrated efficient transduction, expression and regulation of a reporter gene in mice.
Using RNA trans-splicing in htau mice with a lentiviral delivery system to achieve long-term expression of trans-splicing molecules, we have demonstrated efficient correction of endogenous tau mis-splicing in neurons in vitro and in vivo in the brain of adult mice.
Southern blot analysis demonstrated efficient deletion of the floxed allele in the epidermis of tamoxifen-treated Map3k7fl/flK14-Cre-ERT2 mice (Fig. 2).
Together with the development of procedures for purification and concentration, we have demonstrated efficient gene delivery of the luc reporter gene, constructed as described here, to the liver in mice (Fig. 1C in ref. [58]).
These results demonstrated efficient visual individual recognition in cattle achieved using 2D-images.
In vitro experiments demonstrated efficient killing of vTK expressing hepatoma cells after GCV treatment.
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